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For both biocatalyzed anode and cathode you can use a minimal medium which contains all macro (N, P, K) and micro (SO4, metals,...) elements. 
Supplementary to these macro- and micronutrients in the anode an organic substrate is added (e.g. 1 g acetate/L) and an electron acceptor is added in the cathode (continuous bubbling with O2 for oxygenated MFCs or NO3 for denitrifying BES). 
Finally, add a pH buffer compount to avoid dramatic pH changes (e.g. PO4 buffer). 
Go and look for the complete composition of minimale media in one of the publications under the publications section.

The following are the list of quries I have, please anyone find some time to reply me... 
 
1) Can Polarization using Ag/AgCl electrode been done for an MFC using a Potentiostat. If so, is that Linear Sweep Voltammetry technique to be used or any other method. 
 
2) how to analyze completely the cyclic voltammetry graphs

What is the best broth for MFCs?

what is the best medium for MFC (in anode part) ?

The medium affects electron transfer in different ways. 
1. The salinity of the medium will affect the resistance to electron transfer. In general, higher salinity will improve electron transfer. 
2. The pH buffer capacity of your medium has to be high enough (e.g. 60 mmol/L PO4) to counterbalance the pH gradient that will arise, especially in dual chamber BESs. 
3. The medium richness will partly determine your community. One has to guarantee enough micro nutrients to enable microbial metabolism, production of microbial redoxmediators,... However a non-selective medium (like LB) will enable non-electrogens to grow, which will reduce coulombmetric efficiency. Besides, a cheaper and more easy medium is also preferred for sustainable BES operation.

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