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Nafion membranes are often used in two-chamber MFCs but have two disadvantages. They are more expensive than agar salt bridges and have to be regenerated frequently by boiling in H2O2 (30%), then deionized water, 0.5 M H2SO4, and deionized water (each time for 1 h). 
To avoid the use of either Nafion or other membranes researchers try to build single-chamber MFCs or operate the two-chamber MFC in a loop-configuration (Rozendal et al., 2008). In a loop configuration, the effluent from the anode chamber does not leave the system, which is the case in a more traditional configuration: instead, it is directed to the cathode chamber. This largely solves the problem of the membrane pH gradient because the protons that are produced in the anode reaction are actively transported to the cathode chamber, where they compensate for the protons that are consumed in the cathode reaction and retain the the pH in the cathode chamber at a lower value.

Salt bridges are not used in most research MFC's. A Nafion membrane is used instead. (Nafion is a sulfonate-derivatized tetrafluoroethylene that functions as a cation-exchange medium. You could call it a sulfonated Teflon, because both Teflon and Nafion are made by Du Pont.) 
The advantage of Nafion is that it has a lower resistance than a salt bridge.

hello.. 
may be this web pages can be useful  
look into 
http://www.warneronline.com/pdf/wh itepapers/agar_bridges.pdf 
and 
En reacciones en las que no interviene Ag+ u otras especies 
que reaccionarían con Cl-, el electrolito del puente salino 
suele ser KCl. 
¾ Un puente salino típico se prepara calentando 3 g de agar y 
100 g de KCl en 100 ml de agua, hasta que se obtiene una 
disolución transparente. 
¾ Se vierte la disolución en el tubo en U, y se deja que 
gelifique. 
¾ El puente se guarda en contacto con una disolución acuosa 
saturada de KCl. 
good luck friend i´m Luis Alberto

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